Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation

Yujia Sun, Chung Yi Nien, Kai Chen, Hsiao Yun Liu, Jeff Johnston, Julia Zeitlinger, Christine Rushlow

研究成果: 雜誌貢獻期刊論文同行評審

91 引文 斯高帕斯(Scopus)

摘要

The Drosophila genome activator Vielfaltig (Vfl), also known as Zelda (Zld), is thought to prime enhancers for activation by patterning transcription factors (TFs). Such priming is accompanied by increased chromatin accessibility, but the mechanisms by which this occurs are poorly understood. Here, we analyze the effect of Zld on genome-wide nucleosome occupancy and binding of the patterning TF Dorsal (Dl). Our results show that early enhancers are characterized by an intrinsically high nucleosome barrier. Zld tackles this nucleosome barrier through local depletion of nucleosomes with the effect being dependent on the number and position of Zld motifs. Without Zld, Dl binding decreases at enhancers and redistributes to open regions devoid of enhancer activity. We propose that Zld primes enhancers by lowering the high nucleosome barrier just enough to assist TFs in accessing their binding motifs and promoting spatially controlled enhancer activation if the right patterning TFs are present. We envision that genome activators in general will utilize this mechanism to activate the zygotic genome in a robust and precise manner.

原文???core.languages.en_GB???
頁(從 - 到)1703-1714
頁數12
期刊Genome Research
25
發行號11
DOIs
出版狀態已出版 - 11月 2015

指紋

深入研究「Zelda overcomes the high intrinsic nucleosome barrier at enhancers during Drosophila zygotic genome activation」主題。共同形成了獨特的指紋。

引用此