Using biophysics to monitor the essential protonmotive force in bacteria

Mei Ting Chen, Chien Jung Lo

研究成果: 書貢獻/報告類型篇章同行評審

6 引文 斯高帕斯(Scopus)

摘要

Protonmotive force is an essential biological energy format in all levels of cells. Protonmotive force comprises electrical and chemical potential difference across biological membrane. In bacteria, protonmotive force couples to metabolism and ATP production. Moreover, protonmotive force directly provides driving energy of bacterial flagellar motor that is critical for bacterial motility and infection. Due to the small size of bacterial cells, there were limited experimental tools to measure protonmotive force in bacteria. Recent developments of optical membrane potential and intracellular pH indicators provide valuable information on bacterial studies. These new biophysical techniques allow us to monitor the protonmotive force even in single bacterial cell level that shed the light of next generation single-cell physiological experiments towards the understanding of bacterial infection process.

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主出版物標題Advances in Experimental Medicine and Biology
發行者Springer New York LLC
頁面69-79
頁數11
DOIs
出版狀態已出版 - 2016

出版系列

名字Advances in Experimental Medicine and Biology
915
ISSN(列印)0065-2598
ISSN(電子)2214-8019

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