Unveiling the topographic cue rendered by micropatterns for steering cell differentiation by using extrinsic photobiomodulation

Guan Ying Tsai, Thipwadee Klom-In, Meng Jiy Wang, Szu yuan Chen

研究成果: 雜誌貢獻期刊論文同行評審

摘要

It is known that cell culture micropatterns have the ability to facilitate stem cell differentiation induced by specialized chemical factors and different differentiation directions have different optimal micropattern shapes. In this study, by utilizing extrinsic photobiomodulation (EPM) with verteporfin as photosensitizer and light irradiation of 690 nm wavelength as a universal, unbiased, and synchronizing way of inducing differentiation of human umbilical cord Wharton's Jelly mesenchymal stem cells (WJ-MSCs), the topographic cue for cell type specification conveyed in microislands is investigated. It is found that the topographic cues are encoded in the symmetry and aspect ratio of microislands and conformation of cells to microislands is necessary for acquiring the cue. F-actin vertical columns form and cell thickness increases for cells on microislands, and the two effects are enhanced by EPM and correlate with cell differentiation. EPM treatment poises cells in a stationary state to initiate differentiation and the process of making commitment takes two days. Our findings reveal the way to fully exploit topographic cues for promoting and controlling cell differentiation.

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文章編號100229
期刊Journal of Photochemistry and Photobiology
20
DOIs
出版狀態已出版 - 4月 2024

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