Unified description of compressive modulus revealing multiscale mechanics of living cells

Dongshi Guan, Yusheng Shen, Rui Zhang, Pingbo Huang, Pik Yin Lai, Penger Tong

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

How to accurately characterize the modulus of living cells at the whole-cell level with a well-defined measurement geometry and precise mathematical modeling of viscoelastic relaxation is an ongoing challenge in biophysics and mechanobiology. Here, we report combined atomic-force-microscopy (AFM) measurements of stress relaxation and indentation force for 10 cell types ranging from epithelial, muscle, and neuronal cells to blood and stem cells, from which we obtain a unified quantitative description of the compressive modulus E(t) of individual living cells. The cell modulus E(t) is found to have an initial exponential decay at short times t followed by a long-time power-law decay together with a persistent modulus. The three components of E(t) at different timescales thus provide a digital spectrum of mechanical readouts that are closely linked to the hierarchical structure and active stress of living cells. This work provides a reliable experimental framework that can be utilized to characterize the mechanical state of living cells and investigate their physiological functions and diseased states.

原文???core.languages.en_GB???
文章編號043166
期刊Physical Review Research
3
發行號4
DOIs
出版狀態已出版 - 12月 2021

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