Unbinding of the streptavidin-biotin complex by atomic force microscopy: A hybrid simulation study

Jian Zhou, Luzheng Zhang, Yongsheng Leng, Heng Kwong Tsao, Yu Jane Sheng, Shaoyi Jiang

研究成果: 雜誌貢獻期刊論文同行評審

28 引文 斯高帕斯(Scopus)

摘要

A hybrid molecular simulation technique, which combines molecular dynamics and continuum mechanics, was used to study the single-molecule unbinding force of a streptavidin-biotin complex. The hybrid method enables atomistic simulations of unbinding events at the millisecond time scale of atomic force microscopy (AFM) experiments. The logarithmic relationship between the unbinding force of the streptavidin-biotin complex and the loading rate (the product of cantilever spring constant and pulling velocity) in AFM experiments was confirmed by hybrid simulations. The unbinding forces, cantilever and tip positions, locations of energy barriers, and unbinding pathway were analyzed. Hybrid simulation results from this work not only interpret unbinding AFM experiments but also provide detailed molecular information not available in AFM experiments.

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文章編號104905
期刊Journal of Chemical Physics
125
發行號10
DOIs
出版狀態已出版 - 2006

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