Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection

Hsing Ying Lin, Chen Han Huang, Shin Huei Chen, Yu Chia Liu, Wei Zhe Chang, Lai Kwan Chau

研究成果: 雜誌貢獻期刊論文同行評審

6 引文 斯高帕斯(Scopus)

摘要

A novel tubular waveguide particle plasmon resonance (TW-PPR) sensor is demonstrated for label-free biochemical detection. The sensor itself is a microchamber of a defined sample volume, a mechanical support for sensor coating, a waveguide to provide evanescent wave interrogation, and it can be easily extended to a multi-channel format. The sensor resolution is estimated to be 2.6×10-6RIU in measuring solutions of various refractive indices. The sensing system can perform multiple measurements simultaneously and its limit of detection for anti-DNP antibody and streptavidin is 1.2×10-10g/ml (0.55pM) and 2.3×10-10g/ml (3.5pM), respectively. Accurate determination of these analytes with known concentration spiked in artificial urine were examined and the bias is less than ±7%, supporting the utility of the device for analyte screening in more complex media. The TW-PPR sensor can be inexpensively fabricated and has a special niche for monitoring biomolecular interactions in real-time, hence it is ideally suitable for disposable uses, especially promising for convenient high-throughput biochemical sensing applications.

原文???core.languages.en_GB???
頁(從 - 到)268-274
頁數7
期刊Biosensors and Bioelectronics
41
發行號1
DOIs
出版狀態已出版 - 15 3月 2013

指紋

深入研究「Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection」主題。共同形成了獨特的指紋。

引用此