Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

An Cheng Liu, Liang Yu Chen, Chung Fan Chiou, Hung Ju Richard Su, Yu Chia Chan, Pei Hsuan Tasi, Shean Jen Chen, Wen Yih Chen

研究成果: 雜誌貢獻期刊論文同行評審

2 引文 斯高帕斯(Scopus)

摘要

This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.

原文???core.languages.en_GB???
頁(從 - 到)83-87
頁數5
期刊Thermochimica Acta
433
發行號1-2
DOIs
出版狀態已出版 - 1 8月 2005

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