@article{b76f7d268fc946d98a3ca30aa7374e7c,
title = "Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry",
abstract = "This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.",
keywords = "DNA hybridization, Enthalpy, Gene chip, Isothermal titration calorimetry",
author = "Liu, {An Cheng} and Chen, {Liang Yu} and Chiou, {Chung Fan} and Su, {Hung Ju Richard} and Chan, {Yu Chia} and Tasi, {Pei Hsuan} and Chen, {Shean Jen} and Chen, {Wen Yih}",
note = "Funding Information: The authors would like to thank the National Science Council of the Republic of China, Taiwan under Contract No. NSC 92-2214-E-008-003, and the Biomedical Engineering Center, Industrial Technology Research Institute of Taiwan for financially supporting this research.",
year = "2005",
month = aug,
day = "1",
doi = "10.1016/j.tca.2005.01.072",
language = "???core.languages.en_GB???",
volume = "433",
pages = "83--87",
journal = "Thermochimica Acta",
issn = "0040-6031",
number = "1-2",
}