The optical measurement and quantitative analysis of algesia in spodoptera litura larva

Ying Yun Chen, Rong Seng Chang, Mi Yin Tsai, Der Chin Chen

研究成果: 雜誌貢獻期刊論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

Muscle vibration measurement has long been an unique scientific study, in general, and the direct reaction of animals to feel pain (algesia), either from vascular or muscle contraction, is a complex perceptual experience. Thus this paper proposes a way to measure animal algesia quantitatively, by measuring the changes in muscle vibration due to a pinprick on the surface of the skin of a Spodoptera litura larva. Using the laser optical triangulation measurement principle, along with a CMOS image sensor, linear laser, software analysis, and other tools, we quantify the subtle object point displacement, with a precision of up to 10 μm, for our chosen Spodoptera litura larva animal model, in which it is not easy to identify the tiny changes in muscle contraction dynamics with the naked eye. We inject different concentrations of formalin reagent (empty needle, 12% formalin, and 37% formalin) to obtain a variety of different muscle vibration frequencies as the experimental results. Because of the high concentrations of reagent applied, we see a high frequency shift of muscle vibration, which can be presented as pain indices, so that the algesia can be quantified.

原文???core.languages.en_GB???
頁(從 - 到)169-174
頁數6
期刊Journal of the Optical Society of Korea
19
發行號2
DOIs
出版狀態已出版 - 2015

指紋

深入研究「The optical measurement and quantitative analysis of algesia in spodoptera litura larva」主題。共同形成了獨特的指紋。

引用此