The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells

Ching Hsein Chen, Tsan Zon Liu, Chin Hui Chen, Chung Hang Wong, Chi Hung Chen, Fung Jou Lu, Ssu Ching Chen

研究成果: 雜誌貢獻期刊論文同行評審

76 引文 斯高帕斯(Scopus)

摘要

There is increasing evidence that reactive oxygen species (ROS) are intimately involved in the oxidative damage of tissues for a wide variety of pulmonary diseases. Thus, it is desirable to search for chemopreventive agents that can counteract ROS-mediated injury to the pulmonary tissues. Using a human lung fibroblast IMR-90 cells as the experimental model, we first demonstrated that nearly 90% of intracellular ROS could be removed when H2O 2-treated cells (200 μM) simultaneously incubated with 10 μg/mL of tannic acid (TA), gallic acid (GA), ellagic acid (EA), and propyl gallate (PA). Using C11-BODIPY581/591 as a lipid peroxidation probe, we also attested that all these compounds examined (10 μg/mL) could alleviate H2O2-evoked lipid peroxidation phenomena. Next, we examined the protective effects of these compounds on the depletion of intracellular glutathione (iGSH) in H2O2-treated cells using CMF-DA probe. Interestingly, PA was demonstrated to be the only compound that could effectively protect the integrity of iGSH from being depleted by this system. Finally, the protective effects of these compounds against oxidative DNA damage were evaluated using 8-oxoguanine formation as a marker. Our data indicated that all four compounds suppressed the formation of 8-oxoguanine effectively. Taken together, our data suggested that TA, GA, EA, and PA can protect cells from oxidative stress.

原文???core.languages.en_GB???
頁(從 - 到)962-968
頁數7
期刊Molecular Nutrition and Food Research
51
發行號8
DOIs
出版狀態已出版 - 8月 2007

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