The control of alginate degradation to dynamically manipulate scaffold composition for in situ transfection application

Wei Wen Hu, Zhe Chen Hu

研究成果: 雜誌貢獻期刊論文同行評審

13 引文 斯高帕斯(Scopus)

摘要

In this study, nanofibrous scaffolds were used for in situ transfection application. Polyethylenimine (PEI)/DNA complexes adsorbed to alginate nanofibers, so the more alginate fibers resulted in the higher transfection efficiency. However, alginate was not favorable for cell adhesion. Therefore, poly (ε‑caprolactone) (PCL) nanofibers were electrospun with alginate to improve biocompatibility. The in situ transfection results demonstrated that although the incorporated PCL fibers effectively improved cell morphology, the bioactivity and proliferation rates of surface cells were not significantly increased due to the high ratio of alginate fibers. However, the reduction of the alginate ratio may decrease transfection efficiency because the immobilization of nonviral vectors linearly depended on the density of alginate fibers. To maintain transfection efficiency and increase biocompatibility, the stability of alginate fibers were manipulated by adjusting the concentrations of calcium ions during crosslinking. These partially crosslinked alginate fibers were initially intact to allow nanoparticle adsorption for cell uptake, and then gradually degraded in days to create an appropriate environment for cell survival. This dynamic system successfully fulfilled the requirements of both gene delivery and biocompatibility. To our knowledge, this study may be the first one which dynamically regulates scaffold composition for substrate-mediated gene delivery.

原文???core.languages.en_GB???
頁(從 - 到)1169-1178
頁數10
期刊International Journal of Biological Macromolecules
117
DOIs
出版狀態已出版 - 1 10月 2018

指紋

深入研究「The control of alginate degradation to dynamically manipulate scaffold composition for in situ transfection application」主題。共同形成了獨特的指紋。

引用此