TY - JOUR
T1 - Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology
AU - Chen, Chien Cheng
AU - Wong, Tzyy Yue
AU - Chin, Tzu Yun
AU - Lee, Wen Hsien
AU - Kuo, Chan Yen
AU - Hsu, Yi Chiung
N1 - Publisher Copyright:
© Chen et al.
PY - 2020/8/31
Y1 - 2020/8/31
N2 - Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 87, 32, 29, and 28 genes were differentially expressed after 1, 4, 12, 24, and 48 hours of stretching. Furthermore, 10 of the 29 genes that were up-regulated and 11 of the 28 that were down-regulated after 24 h showed the same changes after 48 h. We then examined expression of the genes that encode serpin family E member 1 (SERPINE1), DNA-binding protein inhibitor 1 (ID1), DNA-binding protein inhibitor 3 (ID3), and CCL2, a cytokine that acts as chemotactic factor in monocytes, in an RT-PCR experiment. The same changes were observed for all four genes after all cyclic stretching durations, confirming the NGS results. Taken together, these findings suggest that cyclical stretching can alter cardiac cell physiology by activating cardiac cell metabolism and impacting cholesterol biosynthesis signaling.
AB - Although mechanical forces are involved in pressure-overloaded cardiomyopathy, their effects on gene transcription profiles are not fully understood. Here, we used next-generation sequencing (NGS) to investigate changes in genomic profiles after cyclic mechanical stretching of human cardiomyocytes. We found that 85, 87, 32, 29, and 28 genes were differentially expressed after 1, 4, 12, 24, and 48 hours of stretching. Furthermore, 10 of the 29 genes that were up-regulated and 11 of the 28 that were down-regulated after 24 h showed the same changes after 48 h. We then examined expression of the genes that encode serpin family E member 1 (SERPINE1), DNA-binding protein inhibitor 1 (ID1), DNA-binding protein inhibitor 3 (ID3), and CCL2, a cytokine that acts as chemotactic factor in monocytes, in an RT-PCR experiment. The same changes were observed for all four genes after all cyclic stretching durations, confirming the NGS results. Taken together, these findings suggest that cyclical stretching can alter cardiac cell physiology by activating cardiac cell metabolism and impacting cholesterol biosynthesis signaling.
KW - Cardiac cell
KW - Cyclic stretching
KW - Functional enrichment
KW - Next-generation sequencing
UR - http://www.scopus.com/inward/record.url?scp=85090433683&partnerID=8YFLogxK
U2 - 10.18632/aging.103465
DO - 10.18632/aging.103465
M3 - 期刊論文
C2 - 32759460
AN - SCOPUS:85090433683
SN - 1945-4589
VL - 12
SP - 16035
EP - 16045
JO - Aging
JF - Aging
IS - 16
ER -