Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors

Chih Cheng Huang; Geng Yen Lee; Jen Inn Chyi; Hui Teng Cheng; Chen Pin Hsu; You Ren Hsu; Fan Ren; Yu Lin Wang

doi:10.1149/05006.0223ecst

Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors

Chih Cheng Huang, Geng Yen Lee, Jen Inn Chyi, Hui Teng Cheng, Chen Pin Hsu, You Ren Hsu, Fan Ren, Yu Lin Wang

研究成果: 書貢獻/報告類型 › 會議論文篇章 › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Antibody-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) were used for detecting peptides and revealing the number of binding sites of the antibody and the dissociation constants. Two binding sites were found on this antibody for the peptide and the dissociation constants of the antibody-peptide complex were extracted from a two-binding-site model. The estimated dissociation constants are consistent with the detection limit of the sensor showing the important role of the dissociation constants in the performance of the sensors. The results demonstrate that the AlGaN/GaN HEMTs cannot only be used for biosensors, but also can be used for the biological affinity study.

原文	???core.languages.en_GB???
主出版物標題	Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012
發行者	Electrochemical Society Inc.
頁面	223-232
頁數	10
版本	6
ISBN（列印）	9781607683544
DOIs	https://doi.org/10.1149/05006.0223ecst
出版狀態	已出版 - 2013
事件	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 - Honolulu, HI, United States 持續時間: 7 10月 2012 → 12 10月 2012

出版系列

名字	ECS Transactions
號碼	6
卷	50
ISSN（列印）	1938-5862
ISSN（電子）	1938-6737

???event.eventtypes.event.conference???

???event.eventtypes.event.conference???	Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012
國家/地區	United States
城市	Honolulu, HI
期間	7/10/12 → 12/10/12

存取文件

10.1149/05006.0223ecst

引用此

Huang, C. C., Lee, G. Y., Chyi, J. I., Cheng, H. T., Hsu, C. P., Hsu, Y. R., Ren, F., & Wang, Y. L. (2013). Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012 (6 編輯, 頁 223-232). (ECS Transactions; 卷 50, 編號 6). Electrochemical Society Inc.. https://doi.org/10.1149/05006.0223ecst

@inproceedings{57f12bad412242f29d6f9776e2c5557a,

title = "Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors",

abstract = "Antibody-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) were used for detecting peptides and revealing the number of binding sites of the antibody and the dissociation constants. Two binding sites were found on this antibody for the peptide and the dissociation constants of the antibody-peptide complex were extracted from a two-binding-site model. The estimated dissociation constants are consistent with the detection limit of the sensor showing the important role of the dissociation constants in the performance of the sensors. The results demonstrate that the AlGaN/GaN HEMTs cannot only be used for biosensors, but also can be used for the biological affinity study.",

author = "Huang, {Chih Cheng} and Lee, {Geng Yen} and Chyi, {Jen Inn} and Cheng, {Hui Teng} and Hsu, {Chen Pin} and Hsu, {You Ren} and Fan Ren and Wang, {Yu Lin}",

year = "2013",

doi = "10.1149/05006.0223ecst",

language = "???core.languages.en_GB???",

isbn = "9781607683544",

series = "ECS Transactions",

publisher = "Electrochemical Society Inc.",

number = "6",

pages = "223--232",

booktitle = "Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012",

edition = "6",

note = "Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012 ; Conference date: 07-10-2012 Through 12-10-2012",

}

Huang, CC, Lee, GY, Chyi, JI, Cheng, HT, Hsu, CP, Hsu, YR, Ren, F & Wang, YL 2013, Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 edn, ECS Transactions, 編號 6, 卷 50, Electrochemical Society Inc., 頁 223-232, Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012, Honolulu, HI, United States, 7/10/12. https://doi.org/10.1149/05006.0223ecst

Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors. / Huang, Chih Cheng; Lee, Geng Yen; Chyi, Jen Inn 等.
Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6. 編輯 Electrochemical Society Inc., 2013. p. 223-232 (ECS Transactions; 卷 50, 編號 6).

研究成果: 書貢獻/報告類型 › 會議論文篇章 › 同行評審

TY - GEN

T1 - Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors

AU - Huang, Chih Cheng

AU - Lee, Geng Yen

AU - Chyi, Jen Inn

AU - Cheng, Hui Teng

AU - Hsu, Chen Pin

AU - Hsu, You Ren

AU - Ren, Fan

AU - Wang, Yu Lin

PY - 2013

Y1 - 2013

N2 - Antibody-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) were used for detecting peptides and revealing the number of binding sites of the antibody and the dissociation constants. Two binding sites were found on this antibody for the peptide and the dissociation constants of the antibody-peptide complex were extracted from a two-binding-site model. The estimated dissociation constants are consistent with the detection limit of the sensor showing the important role of the dissociation constants in the performance of the sensors. The results demonstrate that the AlGaN/GaN HEMTs cannot only be used for biosensors, but also can be used for the biological affinity study.

AB - Antibody-immobilized AlGaN/GaN high electron mobility transistors (HEMTs) were used for detecting peptides and revealing the number of binding sites of the antibody and the dissociation constants. Two binding sites were found on this antibody for the peptide and the dissociation constants of the antibody-peptide complex were extracted from a two-binding-site model. The estimated dissociation constants are consistent with the detection limit of the sensor showing the important role of the dissociation constants in the performance of the sensors. The results demonstrate that the AlGaN/GaN HEMTs cannot only be used for biosensors, but also can be used for the biological affinity study.

UR - http://www.scopus.com/inward/record.url?scp=84885749365&partnerID=8YFLogxK

U2 - 10.1149/05006.0223ecst

DO - 10.1149/05006.0223ecst

M3 - 會議論文篇章

AN - SCOPUS:84885749365

SN - 9781607683544

T3 - ECS Transactions

SP - 223

EP - 232

BT - Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012

PB - Electrochemical Society Inc.

T2 - Symposia on Low-Dimensional Nanoscale Electronic and Photonic Devices 5 and State-of-the-Art Program on Compound Semiconductors 2012, SOTAPOCS 54 - 222nd ECS Meeting/PRiME 2012

Y2 - 7 October 2012 through 12 October 2012

ER -

Huang CC, Lee GY, Chyi JI, Cheng HT, Hsu CP, Hsu YR 等. Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors. 於 Low-Dimensional Nanoscale Electronic and Low-Dimensional Nanoscale Electronic and on Compound Semiconductors 54, SOTAPOCS 2012. 6 編輯 Electrochemical Society Inc. 2013. p. 223-232. (ECS Transactions; 6). doi: 10.1149/05006.0223ecst

Study of protein-peptide binding affinity using AlGaN/GaN high electron mobility transistors

摘要

出版系列

???event.eventtypes.event.conference???

存取文件

指紋

引用此