Stem cell culture and differentiation in 3-D scaffolds

Yasuhiko Tabata, Idaszek Joanna, Akon Higuchi

研究成果: 書貢獻/報告類型篇章同行評審

摘要

Conventional two-dimensional (2-D) cultivation are easy to utilize for human pluripotent stem (hPS) cell cultivation in standard techniques and are important for analysis or development of the signal pathways to keep pluripotent state of hPS cells cultivated on 2-D cell culture materials. However, the most efficient protocol to prepare hPS cells is the cell culture in a three dimensional (3-D) cultivation unit because huge numbers of hPS cells should be utilized in clinical treatment. Some 3-D cultivation strategies for hPS cells are considered: (a) microencapsulated cell cultivation in suspended hydrogels, (b) cell cultivation on microcarriers (MCs), (c) cell cultivation on self-aggregated spheroid [cell aggregates; embryoid bodies (EBs) and organoids], (d) cell cultivation on microfibers or nanofibers, and (e) cell cultivation in macroporous scaffolds. These cultivation ways are described in this chapter.

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主出版物標題Progress in Molecular Biology and Translational Science
編輯Akon Higuchi, Yinghong Zhou, Shih-Hwa Chiou
發行者Elsevier B.V.
頁面109-127
頁數19
ISBN(列印)9780443134111
DOIs
出版狀態已出版 - 1月 2023

出版系列

名字Progress in Molecular Biology and Translational Science
199
ISSN(列印)1877-1173
ISSN(電子)1878-0814

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