SPR bacterial pathogen biosensor: The importance of fluidic conditions and probing depth

Chun Jen Huang, Wolfgang Knoll, Angela Sessitsch, Jakub Dostalek

研究成果: 雜誌貢獻期刊論文同行評審

14 引文 斯高帕斯(Scopus)

摘要

The sensitivity of surface plasmon resonance (SPR) biosensor technology for detection of bacterial analytes is investigated as a function of (a) sample flow conditions and (b) depth of probing electromagnetic field. These parameters are extremely important as such analytes exhibit large (of around micrometer) size which significantly hinders their diffusion-driven transfer from a liquid sample to the sensor and their subsequent specific capture by attached recognition elements. This is due to small diffusion coefficient and strong shear stress that decreases the stability of bonds between the bacterium specific epitope and recognition elements immobilized at the sensor surface. The importance of accurate control of sample flow conditions and probing depth in order to maximize SPR sensor response is experimentally demonstrated and supported by an analytical theory. The tuning of the probing depth of surface plasmon evanescent field to match the size of the target analyte is pursued by using long range surface plasmons.

原文???core.languages.en_GB???
頁(從 - 到)166-171
頁數6
期刊Talanta
122
DOIs
出版狀態已出版 - 5月 2014

指紋

深入研究「SPR bacterial pathogen biosensor: The importance of fluidic conditions and probing depth」主題。共同形成了獨特的指紋。

引用此