Serum protein adsorption and platelet adhesion on pluronic™-adsorbed polysulfone membranes

Akon Higuchi, Kaichiro Sugiyama, Boo Ok Yoon, Masaru Sakurai, Mariko Hara, Masaya Sumita, Shu Ichi Sugawara, Takashi Shirai

研究成果: 雜誌貢獻期刊論文同行評審

177 引文 斯高帕斯(Scopus)

摘要

We examined plasma protein adsorption and platelet adhesion to polysulfone (PSf) flat membranes coated with Pluronic™ with varying polyethylene oxide (PEO) block length. Adsorption of albumin, globulin and fibrinogen to Pluronic™-coated PSf membranes was independent of plasma dilution when concentrations of human blood plasma above 20% were applied. Increasing coating concentrations of aqueous Pluronic™ solution resulted in decreased protein adsorption by the PSf membranes. Pluronic™ F68, which was more hydrophilic than Pluronic™ L62 or L64 and had 80% of PEO content, was the most effective at suppressing the adsorption of plasma proteins and platelet adhesion to PSf membranes. We developed a mixed protein solution containing human albumin, γ-globulin and fibrinogen to attempt to mimic the competitive and cooperative binding effects found in plasma. Fibrinogen adsorption from plasma could be recapitulated by the mixed protein solution. The number of platelets adhering to the PSf membranes decreased as the coating concentration of Pluronic™ solution was increased, and platelet adhesion decreased in parallel with fibrinogen adsorption. These results suggest that the bioinert property of PEO segments in the Pluronic™, which is ascribed to their high flexibility in aqueous media, suppresses the adsorption of plasma proteins and platelets to the Pluronic™-coated PSf membranes.

原文???core.languages.en_GB???
頁(從 - 到)3235-3245
頁數11
期刊Biomaterials
24
發行號19
DOIs
出版狀態已出版 - 8月 2003

指紋

深入研究「Serum protein adsorption and platelet adhesion on pluronic™-adsorbed polysulfone membranes」主題。共同形成了獨特的指紋。

引用此