Separation of vascular endothelial cells from peripheral blood through porous polymeric membranes

Masahiro Noda, Yuki Matsuoka, Shizue Hayashi, Akon Higuchi

研究成果: 會議貢獻類型會議論文同行評審

1 引文 斯高帕斯(Scopus)

摘要

Cell separation is a key technology in the isolation of cells from tissue and the transplantation of blood cells or mesenchymal stem cells. Purification and isolation of endothelial cells from cord blood will be useful for the regeneration of veins in clinical applications, because human umbilical vein endothelial cells are reported to exist in the cord blood. In the current studies, we investigated the separation of normal human umbilical vein endothelial cells (HUVEC) from blood cells by filtration through various porous polymeric membranes. We found a better permeation of blood cells than that of HUVEC through polyurethane foaming membranes (pore size = 8.9 and 12-μm). This appears to be due to the relatively bigger cell size of HUVEC. We further found a relatively good separation factor with Nylon net filter membranes in the recovery solution using 20wt% albumin and 10wt% dextran solution. This ability of the Nylon net filter membranes to separate HUVEC and blood cells was due to a sieving effect that results from an optimal pore size.

原文???core.languages.en_GB???
頁面1920
頁數1
出版狀態已出版 - 2006
事件55th SPSJ Annual Meeting - Nagoya, Japan
持續時間: 24 5月 200626 5月 2006

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???event.eventtypes.event.conference???55th SPSJ Annual Meeting
國家/地區Japan
城市Nagoya
期間24/05/0626/05/06

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