Separation of proteins by surface modified polysulfone membranes

Akon Higuchi, Satoko Mishima, Tsutomu Nakagawa

研究成果: 雜誌貢獻期刊論文同行評審

73 引文 斯高帕斯(Scopus)

摘要

Ultrafiltration separation experiments on mixed solution of bovine serum albumin and γ-globulin were performed in a stirred batch cell through unmodified and surface modified polysulfone membranes, γ-Globulin permeated through the surface modified membrane to some extent but the bovine serum albumin was 100% rejected towards the end of the concentration process at pH 7.2 and 9.0, although the molecular weight of γ-globulin is higher than that of bovine serum albumin. The unmodified membrane did not extensively separate the proteins in the mixed solution at any pH, unlike the surface modified membrane. It is suggested that the separation between bovine serum albumin and γ-globulin through the surface modified membranes is caused, not by a sieving effect or by charge repulsion between membranes and proteins, but by the balance of hydrophilic and hydrophobic segments on the surface of the modified membranes.

原文???core.languages.en_GB???
頁(從 - 到)175-185
頁數11
期刊Journal of Membrane Science
57
發行號2-3
DOIs
出版狀態已出版 - 15 4月 1991

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