Separation and purification of bone marrow stromal cells using membrane filtration

Y. Gomei, Y. Shindo, T. Mori, T. Uyama, A. Umezawa, A. Higuchi

研究成果: 書貢獻/報告類型會議論文篇章同行評審

摘要

The separation and purification of bone marrow stromal cells from mixed KUSA-A1 and 9-15C cells using membrane filtration were investigated. The aim was to find the optimal membrane selection and filtration conditions for the separation of marrow stromal cells. Base membranes used for the filtration were Nylon mesh filter, sponge polyurethane (PU) porous membranes and sponge PU membranes containing 0.61% of epoxy group. After the cultivation of the mixed cells, appropriate cell concentration was prepared and the mixed or single cell solution was permeated through the membranes. High recovery ratio of 9-15C cells was obtained at the single cell concentration of 5000/ml, while permeation ratios of both 9-15C and KUSA-A1 cells were less than 3%.

原文???core.languages.en_GB???
主出版物標題Transactions - 7th World Biomaterials Congress
頁面895
頁數1
出版狀態已出版 - 2004
事件Transactions - 7th World Biomaterials Congress - Sydney, Australia
持續時間: 17 5月 200421 5月 2004

出版系列

名字Transactions - 7th World Biomaterials Congress

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???event.eventtypes.event.conference???Transactions - 7th World Biomaterials Congress
國家/地區Australia
城市Sydney
期間17/05/0421/05/04

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