Near-field electrospinning (NFES) is capable of precisely deposit one-dimensional (1D) or two-dimensional (2D) highly aligned micro/nano fibers (NMFs) by electrically discharged a polymer solution. In this paper, a new integration of three-dimensional (3D) architectures of NFES electrospun polyvinylidene fluoride (PVDF) NMFs with the 3D printed topologically tailored substrate are demonstrated in a direct-write and in-situ poled manner, called wavy- substrate self-powered sensors (WSS). The fabrication steps are composed of the additive manufacture of 3D printed flexible and sinusoidal wavy substrate, metallization and NFES electrospun fibers in the 3D topology. This 3D architecture is capable of greatly enhancing the piezoelectric output. Finally, the proposed piezoelectrically integrated 3D architecture is applied to the self-powered sensors such as foot pressure measurement, human motion monitoring and finger-induced power generation. The proposed technique demonstrates the advancement of existing electrospinning technologies in constructing 3D structures and several promising applications for biomedical and wearable electronics.