Revisiting the streptavidin-biotin binding by using an aptamer and displacement isothermal calorimetry titration

Tai Chih Kuo, Ching Wei Tsai, Peng Chen Lee, Wen Yih Chen

研究成果: 雜誌貢獻回顧評介論文同行評審

11 引文 斯高帕斯(Scopus)

摘要

The association constant of a well-known streptavidin-biotin binding has only been inferred from separately measured kinetic parameters. In a single experiment, we obtained Ka 1 × 1012 M-1 by using a streptavidin-binding aptamer and ligand-displacement isothermal titration calorimetry. This study explores the challenges of determining thermodynamic parameters and the derived equilibrium binding affinity of tight ligand-receptor binding.

原文???core.languages.en_GB???
頁(從 - 到)125-128
頁數4
期刊Journal of Molecular Recognition
28
發行號3
DOIs
出版狀態已出版 - 3月 2015

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