Redundancy of non-AUG initiators: A clever mechanism to enhance the efficiency of translation in yeast

Kuang Jung Chang, Grace Lin, Lee Chung Men, Chien Chia Wang

研究成果: 雜誌貢獻期刊論文同行評審

21 引文 斯高帕斯(Scopus)

摘要

It was recently shown that ALA1, the only alanyl-tRNA synthetase gene in Saccharomyces cerevisiae, uses two successive ACG triplets as the translation initiators for its mitochondrial form. Evidence presented here argues that the second ACG triplet not only acts as a remedial initiation site for scanning ribosomes that skip the first ACG, but also enhances the activity of the preceding initiator by providing a preferable "A" at its relative position +4. Therefore, ALA1 constructs with redundant ACG initiators exhibit stronger complementing activity and express a higher level of protein than do those with a single ACG initiator. A similar scenario is seen when a single or redundant ACG triplets are placed in the positions of the first and second AUG initiators of VAS1, which serve as the start sites of the mitochondrial and cytoplasmic forms of valyl-tRNA synthetase, respectively. Cumulatively, the results suggest that this feature of redundancy of non-AUG initiators in a single mRNA per se may represent a novel paradigm for improving the efficiency of a poor or otherwise nonproductive initiation event.

原文???core.languages.en_GB???
頁(從 - 到)7775-7783
頁數9
期刊Journal of Biological Chemistry
281
發行號12
DOIs
出版狀態已出版 - 24 3月 2006

指紋

深入研究「Redundancy of non-AUG initiators: A clever mechanism to enhance the efficiency of translation in yeast」主題。共同形成了獨特的指紋。

引用此