Recognition of substrates by membrane potential of immobilized glucose oxidase membranes

Akon Higuchi, Mariko Hara, Kyu‐Sik ‐S Yun, Tae‐Moon ‐M Tak

研究成果: 雜誌貢獻期刊論文同行評審

10 引文 斯高帕斯(Scopus)

摘要

The shifts in membrane potential, caused by the injection of glucose into a permeation cell, were measured using immobilized (entrapped) glucose oxidase membranes. No, pH change in the permeation cell was observed upon injection of glucose, but the shift in membrane potential was definitely detected. The shift in membrane potential was observed under nitrogen bubbling (in the absence of oxygen) using initially used enzyme membranes. It was, therefore, suggested that the shifts in membrane potential were not caused by an enzyme‐substrate reaction, but by binding of the substrate to the enzyme, which indues a conformational change in the enzyme and leads to a change in charge density in the enzyme membrane. This mechanism is also supported by the fact that the shifts in membrane potential were observed upon injection of not only D‐glucose but also L‐glucose as reported in our previous study [J. Chem. Soc. Faraday Trans., 87, 695 (1991)]. © 1994 John Wiley & Sons, Inc.

原文???core.languages.en_GB???
頁(從 - 到)1735-1739
頁數5
期刊Journal of Applied Polymer Science
51
發行號10
DOIs
出版狀態已出版 - 7 3月 1994

指紋

深入研究「Recognition of substrates by membrane potential of immobilized glucose oxidase membranes」主題。共同形成了獨特的指紋。

引用此