Rapid identification of health care-associated infections with an integrated fluorescence anisotropy system

Ki Soo Park, Chen Han Huang, Kyungheon Lee, Yeong Eun Yoo, Cesar M. Castro, Ralph Weissleder, Hakho Lee

研究成果: 雜誌貢獻期刊論文同行評審

43 引文 斯高帕斯(Scopus)

摘要

Health care-associated infections (HAIs) and drug-resistant pathogens have become a major health care issue with millions of reported cases every year. Advanced diagnostics would allow clinicians to more quickly determine the most effective treatment, reduce the nonspecific use of broad-spectrum antimicrobials, and facilitate enrollment in new antibiotic treatments. We present a new integrated system, polarization anisotropy diagnostics (PAD), for rapid detection of HAI pathogens. The PAD uses changes of fluorescence anisotropy when detection probes recognize target bacterial nucleic acids. The technology is inherently robust against environmental noise and economically scalable for parallel measurements. The assay is fast (2 hours) and performed on-site in a single-tube format. When applied to clinical samples obtained from interventional procedures, the PAD determined the overall bacterial burden, differentiated HAI bacterial species, and identified drug resistance and virulence status. The PAD system holds promise as a powerful tool for near-patient, rapid HAI testing.

原文???core.languages.en_GB???
文章編號e1600300
期刊Science Advances
2
發行號5
DOIs
出版狀態已出版 - 5月 2016

指紋

深入研究「Rapid identification of health care-associated infections with an integrated fluorescence anisotropy system」主題。共同形成了獨特的指紋。

引用此