TY - JOUR
T1 - Rapid biosynthesis of silver nanoparticles using Crotalaria verrucosa leaves against the dengue vector Aedes aegypti
T2 - What happens around? An analysis of dragonfly predatory behaviour after exposure at ultra-low doses
AU - Murugan, Kadarkarai
AU - Sanoopa, C. P.
AU - Madhiyazhagan, Pari
AU - Dinesh, Devakumar
AU - Subramaniam, Jayapal
AU - Panneerselvam, Chellasamy
AU - Roni, Mathath
AU - Suresh, Udaiyan
AU - Nicoletti, Marcello
AU - Alarfaj, Abdullah A.
AU - Munusamy, Murugan A.
AU - Higuchi, Akon
AU - Kumar, Suresh
AU - Perumalsamy, Haribalan
AU - Ahn, Young Joon
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2015 Taylor & Francis.
PY - 2016/4/2
Y1 - 2016/4/2
N2 - Aedes aegypti is a primary vector of dengue, a mosquito-borne viral disease infecting 50-100 million people every year. Here, we biosynthesised mosquitocidal silver nanoparticles (AgNP) using the aqueous leaf extract of Crotalaria verrucosa. The green synthesis of AgNP was studied by UV-vis spectroscopy, SEM, EDX and FTIR. C. verrucosa-synthesised AgNPs were toxic against A. aegypti larvae and pupae. LC50 of AgNP ranged from 3.496 ppm (I instar larvae) to 17.700 ppm (pupae). Furthermore, we evaluated the predatory efficiency of dragonfly nymphs, Brachydiplax sobrina, against II and III instar larvae of A. aegypti in an aquatic environment contaminated with ultra-low doses of AgNP. Under standard laboratory conditions, predation after 24 h was 87.5% (II) and 54.7% (III). In an AgNP-contaminated environment, predation was 91 and 75.5%, respectively. Overall, C. verrucosa-synthesised AgNP could be employed at ultra-low doses to reduce larval population of dengue vectors enhancing predation rates of dragonfly nymphs.
AB - Aedes aegypti is a primary vector of dengue, a mosquito-borne viral disease infecting 50-100 million people every year. Here, we biosynthesised mosquitocidal silver nanoparticles (AgNP) using the aqueous leaf extract of Crotalaria verrucosa. The green synthesis of AgNP was studied by UV-vis spectroscopy, SEM, EDX and FTIR. C. verrucosa-synthesised AgNPs were toxic against A. aegypti larvae and pupae. LC50 of AgNP ranged from 3.496 ppm (I instar larvae) to 17.700 ppm (pupae). Furthermore, we evaluated the predatory efficiency of dragonfly nymphs, Brachydiplax sobrina, against II and III instar larvae of A. aegypti in an aquatic environment contaminated with ultra-low doses of AgNP. Under standard laboratory conditions, predation after 24 h was 87.5% (II) and 54.7% (III). In an AgNP-contaminated environment, predation was 91 and 75.5%, respectively. Overall, C. verrucosa-synthesised AgNP could be employed at ultra-low doses to reduce larval population of dengue vectors enhancing predation rates of dragonfly nymphs.
KW - Brachydiplax sobrina
KW - FtIr
KW - arbovirus
KW - edX
KW - green synthesis
KW - nanobiotechnologies
KW - seM
UR - http://www.scopus.com/inward/record.url?scp=84960419784&partnerID=8YFLogxK
U2 - 10.1080/14786419.2015.1074230
DO - 10.1080/14786419.2015.1074230
M3 - 期刊論文
C2 - 26284510
AN - SCOPUS:84960419784
SN - 1478-6419
VL - 30
SP - 826
EP - 833
JO - Natural Product Research
JF - Natural Product Research
IS - 7
ER -