TY - JOUR
T1 - Rapid analysis of abused drugs using nanostructured silicon surface assisted laser desorption/ionization mass spectrometry
AU - Cheng, Yu Che
AU - Chen, Kun Han
AU - Wang, Juo Shin
AU - Hsu, Wen Liu
AU - Chien, Chih Cheng
AU - Chen, Wen Yih
AU - Tsao, Chia Wen
PY - 2012/2/7
Y1 - 2012/2/7
N2 - This study developed a rapid, sensitive, and matrix-free method for the determination of amphetamine (AMP), methamphetamine (MA), codeine (COD), morphine (MOR), and ketamine (KET) using nanostructured silicon surface assisted laser desorption/ionization mass spectrometry (nSi-MS). The nanostructured silicon (nSi) chip used in this study was created by employing the metal-assisted etching process. Drug standard tests were applied to the nSi chip platform to evaluate the nSi-MS performance, including detection sensitivity, limit of detection, linearity, and repeatability. Real urine samples obtained from drug addict detainees were directly applied to the nSi chip for drug analysis. By observing the nSi-MS spectra, the target drug peaks can be identified; and an antibody pull-down assay was performed to confirm the specificity of the detected targets. nSi-MS drug quantification was assayed, yielding comparable results with those from using the GC-MS approach. The advantages of applying nSi-MS to analyze AMP, MA, COD, MOR, and KET in the urine of addicts are simple, extremely small urine volumes (∼10 μL), and a fast analysis procedure (<15 minutes).
AB - This study developed a rapid, sensitive, and matrix-free method for the determination of amphetamine (AMP), methamphetamine (MA), codeine (COD), morphine (MOR), and ketamine (KET) using nanostructured silicon surface assisted laser desorption/ionization mass spectrometry (nSi-MS). The nanostructured silicon (nSi) chip used in this study was created by employing the metal-assisted etching process. Drug standard tests were applied to the nSi chip platform to evaluate the nSi-MS performance, including detection sensitivity, limit of detection, linearity, and repeatability. Real urine samples obtained from drug addict detainees were directly applied to the nSi chip for drug analysis. By observing the nSi-MS spectra, the target drug peaks can be identified; and an antibody pull-down assay was performed to confirm the specificity of the detected targets. nSi-MS drug quantification was assayed, yielding comparable results with those from using the GC-MS approach. The advantages of applying nSi-MS to analyze AMP, MA, COD, MOR, and KET in the urine of addicts are simple, extremely small urine volumes (∼10 μL), and a fast analysis procedure (<15 minutes).
UR - http://www.scopus.com/inward/record.url?scp=84862956000&partnerID=8YFLogxK
U2 - 10.1039/c1an15913e
DO - 10.1039/c1an15913e
M3 - 期刊論文
C2 - 22146524
AN - SCOPUS:84862956000
SN - 0003-2654
VL - 137
SP - 654
EP - 661
JO - Analyst
JF - Analyst
IS - 3
ER -