摘要
The production of interferon-β by NB1-RGB fibroblast cells cultured on protein and peptide membranes prepared from silk fibroin, motif peptides of silk fibroin [(AG)n] containing arginine-glycine-aspartic acid (RGD) peptide, and Pronectin™ was investigated. The cell density on various protein and peptide membranes was approximately the same, although the production of interferon-β depended significantly on the membranes where the cells were cultured. The highest production of interferon-β was observed when the cells were cultured on (AG)6RGD(AG)7 membranes prepared with hexafluoroacetone (HFA) as the casting solvent. On RGD-containing peptide membranes more centrally located in the peptides, the cells produced more interferon-β when the peptide membranes were prepared with HFA as the casting solvent. However, there was no enhanced production of interferon-βb by cells on (AG)6RGD(AG)7 membranes prepared with 9 mol/L LiBr or 4.5 mol/L LiClO4 solution as the casting solvent. Therefore, both the chemical composition and the secondary and higher order structure of the peptide membranes are important for enhanced production of interferon-β. The blocking of integrin β1 on the cells by anti-integrin β1 antibody prevented the enhanced production of interferon-β on (AG)6RGD(AG)7 membranes prepared with HFA. We suggest that the cells must bind to the RGD sequence having the appropriate conformation through their integrin β 1 for enhanced production of interferon-β.
原文 | ???core.languages.en_GB??? |
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頁(從 - 到) | 369-378 |
頁數 | 10 |
期刊 | Journal of Biomedical Materials Research - Part A |
卷 | 65 |
發行號 | 3 |
DOIs | |
出版狀態 | 已出版 - 1 6月 2003 |