Production of interferon-β by fibroblast cells on membranes prepared with RGD-containing peptides

Akon Higuchi, Yasunari Takanashi, Nobuya Tsuzuki, Tetsuo Asakura, Chong Su Cho, Toshihiro Akaike, Mariko Hara

研究成果: 雜誌貢獻期刊論文同行評審

22 引文 斯高帕斯(Scopus)

摘要

The production of interferon-β by NB1-RGB fibroblast cells cultured on protein and peptide membranes prepared from silk fibroin, motif peptides of silk fibroin [(AG)n] containing arginine-glycine-aspartic acid (RGD) peptide, and Pronectin™ was investigated. The cell density on various protein and peptide membranes was approximately the same, although the production of interferon-β depended significantly on the membranes where the cells were cultured. The highest production of interferon-β was observed when the cells were cultured on (AG)6RGD(AG)7 membranes prepared with hexafluoroacetone (HFA) as the casting solvent. On RGD-containing peptide membranes more centrally located in the peptides, the cells produced more interferon-β when the peptide membranes were prepared with HFA as the casting solvent. However, there was no enhanced production of interferon-βb by cells on (AG)6RGD(AG)7 membranes prepared with 9 mol/L LiBr or 4.5 mol/L LiClO4 solution as the casting solvent. Therefore, both the chemical composition and the secondary and higher order structure of the peptide membranes are important for enhanced production of interferon-β. The blocking of integrin β1 on the cells by anti-integrin β1 antibody prevented the enhanced production of interferon-β on (AG)6RGD(AG)7 membranes prepared with HFA. We suggest that the cells must bind to the RGD sequence having the appropriate conformation through their integrin β 1 for enhanced production of interferon-β.

原文???core.languages.en_GB???
頁(從 - 到)369-378
頁數10
期刊Journal of Biomedical Materials Research - Part A
65
發行號3
DOIs
出版狀態已出版 - 1 6月 2003

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