Probing Interactions between Metal-Organic Frameworks and Freestanding Enzymes in a Hollow Structure

Sheng Yu Chen, Wei Shang Lo, Yi Da Huang, Xiaomeng Si, Fu Siang Liao, Shang Wei Lin, Benjamin P. Williams, Ting Qian Sun, Hao Wei Lin, Yuanyuan An, Tu Sun, Yanhang Ma, Hsiao Ching Yang, Lien Yang Chou, Fa Kuen Shieh, Chia Kuang Tsung

研究成果: 雜誌貢獻期刊論文同行評審

44 引文 斯高帕斯(Scopus)

摘要

It has been reported that the biological functions of enzymes could be altered when they are encapsulated in metal-organic frameworks (MOFs) due to the interactions between them. Herein, we probed the interactions of catalase in solid and hollow ZIF-8 microcrystals. The solid sample with confined catalase is prepared through a reported method, and the hollow sample is generated by hollowing the MOF crystals, sealing freestanding enzymes in the central cavities of hollow ZIF-8. During the hollowing process, the samples were monitored by small-angle X-ray scattering (SAXS) spectroscopy, electron microscopy, powder X-ray diffraction (PXRD), and nitrogen sorption. The interfacial interactions of the two samples were studied by infrared (IR) and fluorescence spectroscopy. IR study shows that freestanding catalase has less chemical interaction with ZIF-8 than confined catalase, and a fluorescence study indicates that the freestanding catalase has lower structural confinement. We have then carried out the hydrogen peroxide degradation activities of catalase at different stages and revealed that the freestanding catalase in hollow ZIF-8 has higher activity.

原文???core.languages.en_GB???
頁(從 - 到)6630-6635
頁數6
期刊Nano Letters
20
發行號9
DOIs
出版狀態已出版 - 9 9月 2020

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