Prenylated FMN: Biosynthesis, purification, and Fdc1 activation

Anna N. Khusnutdinova, Johnny Xiao, Po Hsiang Wang, Khorcheska A. Batyrova, Robert Flick, Elizabeth A. Edwards, Alexander F. Yakunin

研究成果: 書貢獻/報告類型篇章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Prenylated flavin mononucleotide (prFMN) is a recently discovered flavin cofactor produced by the UbiX family of FMN prenyltransferases, and is required for the activity of UbiD-like reversible decarboxylases. The latter enzymes are known to be involved in ubiquinone biosynthesis and biotransformation of lignin, aromatic compounds, and unsaturated aliphatic acids. However, exploration of uncharacterized UbiD proteins for biotechnological applications is hindered by our limited knowledge about the biochemistry of prFMN and prFMN-dependent enzymes. Here, we describe experimental protocols and considerations for the biosynthesis of prFMN in vivo and in vitro, in addition to cofactor extraction and application for activation of UbiD proteins.

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主出版物標題Methods in Enzymology
編輯Bruce A. Palfey
發行者Academic Press Inc.
頁面469-488
頁數20
ISBN(列印)9780128168295
DOIs
出版狀態已出版 - 2019

出版系列

名字Methods in Enzymology
620
ISSN(列印)0076-6879
ISSN(電子)1557-7988

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