Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen, J. A. Starzmann, C. C. Liu

研究成果: 雜誌貢獻期刊論文同行評審

19 引文 斯高帕斯(Scopus)

摘要

Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

原文???core.languages.en_GB???
頁(從 - 到)971-975
頁數5
期刊Biotechnology and Bioengineering
24
發行號4
DOIs
出版狀態已出版 - 4月 1982

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