Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen; J. A. Starzmann; C. C. Liu

doi:10.1002/bit.260240416

Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

A. K. Chen, J. A. Starzmann, C. C. Liu

材料科學與工程研究所

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

19 引文斯高帕斯（Scopus）

摘要

Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD⁺ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10⁻⁴M to 10⁻¹M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

原文	???core.languages.en_GB???
頁（從 - 到）	971-975
頁數	5
期刊	Biotechnology and Bioengineering
卷	24
發行號	4
DOIs	https://doi.org/10.1002/bit.260240416
出版狀態	已出版 - 4月 1982

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10.1002/bit.260240416

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AU - Starzmann, J. A.

AU - Liu, C. C.

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N2 - Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

AB - Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD+ and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10−4M to 10−1M. The reproducibility of the measurement and the optimal conditions for glycerol determination were described.

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Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase

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