Potentiometric method for subatrate analysis using immobilized NAD+‐dependent oxidoreductase enzymes

A. K. Chen, C. C. Liu, J. G. Schiller

研究成果: 雜誌貢獻期刊論文同行評審

12 引文 斯高帕斯(Scopus)

摘要

Two coenzyme‐dependent oxidoreductases, glucose dehydrogenase and alcohol dehydrogenase, were immobilized in polyacrylamide gel over a platinum grid matrix and used as enzyme electrodes to measure their substrate concentrations in buffered aqueous solutions. The immobilized enzymes were used to oxidize their substrates in the presence of NAD+. Ferricyanide was used as the redox mediator and electroactive specific. The determinations of glucose and ethenol were utilized to demonstrate and evaluate the performance of the system. The described methodology should be readily applicable to the analysis of numerous other substrates of coenzyme‐dependent oxidoreductases.

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頁(從 - 到)1905-1915
頁數11
期刊Biotechnology and Bioengineering
21
發行號11
DOIs
出版狀態已出版 - 11月 1979

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