摘要
DNA aptamers carrying Pt nanoparticles prepared with cisplatin showed peroxidase enzymatic activity while retaining the specific binding ability of the aptamers. Optimal preparation conditions of DNA-Pt complex prepared with cisplatin were investigated on the synthesis at pH 7-11, a reaction time of 1-18 h and 90°C. The enzymatic reaction of DNA-Pt complex obeyed Michaelis-Menten kinetics. KM for the DNA-Pt complex was found to be of the same order as KM for hemin and hemin-DNA complex, but one order of magnitude higher than that of horseradish peroxidase. A sandwich type of DNA enzyme-linked aptamer assay (DLAA) using DNA-Pt complex successively detected target protein of thrombin. DLAA using DNA-Pt complex fractioned by ultrafiltration membranes having a molecular weight cut-off of 30 000 and 300 000 showed 1.9-times higher sensitivity than DLAA using DNA-Pt complex without fraction. The DNA-Pt complex having specific size was effective for the sensitive detection of thrombin in DLAA.
原文 | ???core.languages.en_GB??? |
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頁(從 - 到) | 67-82 |
頁數 | 16 |
期刊 | Journal of Biomaterials Science, Polymer Edition |
卷 | 21 |
發行號 | 1 |
DOIs | |
出版狀態 | 已出版 - 1 1月 2010 |