Patterned networks of mouse hippocampal neurons on peptide-coated gold surfaces

Daniel A. Heller, Veronika Garga, Keith J. Kelleher, Tai Chou Lee, Sunil Mahbubani, Laura A. Sigworth, T. Randall Lee, Michael A. Rea

研究成果: 雜誌貢獻期刊論文同行評審

62 引文 斯高帕斯(Scopus)

摘要

Patterned networks of hippocampal neurons were generated on peptide-coated gold substrates prepared by microscope projection photolithography and microcontact printing. A 19 amino acid peptide fragment of laminin A (PA22-2) that includes the IKVAV cell adhesion domain was used to direct patterns of cell adhesion in primary culture. Microscale grid patterns of peptide were deposited on gold-coated glass cover slips by soft lithography using "stamps" fashioned from polydimethylsiloxane. Strong coordination bonding between gold atoms on the surface and the sulfur atoms of the N-terminal cysteine residues supported stable adhesion of the peptide, which was confirmed by immunofluorescence using anti-IKVAV antiserum. Dispersed hippocampal cells isolated from neonatal mouse pups were grown on peptide-patterned gold substrates for 7 days. Neurons preferentially adhered to peptide-coated regions of the gold surface and restricted their processes to the peptide patterns. Whole cell recordings of neurons grown in patterned arrays revealed an average membrane potential of -50mV, as well as the presence of voltage-gated ion conductances. Peptide-modified gold surfaces serve as convenient and effective substrates for growing ordered neural networks that are compatible with existing multi-electrode array recording technology.

原文???core.languages.en_GB???
頁(從 - 到)883-889
頁數7
期刊Biomaterials
26
發行號8
DOIs
出版狀態已出版 - 3月 2005

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