p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu Ching Chen; Ching Hsein Chen; Chi Liang Chern; Li Sung Hsu; Ya Chun Huang; King Thom Chung; Soi Moi Chye

doi:10.1016/j.tiv.2005.12.003

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

Ssu Ching Chen, Ching Hsein Chen, Chi Liang Chern, Li Sung Hsu, Ya Chun Huang, King Thom Chung, Soi Moi Chye

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31 引文斯高帕斯（Scopus）

摘要

The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨ_m) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

原文	???core.languages.en_GB???
頁（從 - 到）	801-807
頁數	7
期刊	Toxicology in Vitro
卷	20
發行號	6
DOIs	https://doi.org/10.1016/j.tiv.2005.12.003
出版狀態	已出版 - 9月 2006

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10.1016/j.tiv.2005.12.003

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title = "p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells",

abstract = "The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.",

keywords = "Apoptosis, Mardin-Darby canine kidney cells, p-Phenylenediamine, p53",

author = "Chen, {Ssu Ching} and Chen, {Ching Hsein} and Chern, {Chi Liang} and Hsu, {Li Sung} and Huang, {Ya Chun} and Chung, {King Thom} and Chye, {Soi Moi}",

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AU - Chen, Ssu Ching

AU - Chen, Ching Hsein

AU - Chern, Chi Liang

AU - Hsu, Li Sung

AU - Huang, Ya Chun

AU - Chung, King Thom

AU - Chye, Soi Moi

PY - 2006/9

Y1 - 2006/9

N2 - The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

AB - The mechanism of toxicity p-phenylenediamine (p-PD), a component of human permanent hair dye and a suspected carcinogen, on the growth of Mardin-Darby canine kidney cells (MDCK) was investigated. With the analysis of flow cytometry, a dose-dependent accumulation of the sub-G1 peak and the G0/G1-phase arrested in cell cycle, and time-dependent induction of apoptosis after staining with Annexin V-Fluorescein and propidium iodide were observed. After the treatment of cells with p-PD, dose dependent DNA fragmentation shown by gel electrophoresis, the reduction of membrane potential (ΔΨm) by mitochondria membrane depolarization and the increase of the expression of p53 protein in cells, suggested that the effect of p-PD on overall viability and cell numbers is mediated by an increase in apoptosis.

KW - Apoptosis

KW - Mardin-Darby canine kidney cells

KW - p-Phenylenediamine

KW - p53

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U2 - 10.1016/j.tiv.2005.12.003

DO - 10.1016/j.tiv.2005.12.003

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JO - Toxicology in Vitro

JF - Toxicology in Vitro

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ER -

p-Phenylenediamine induces p53-mediated apoptosis in Mardin-Darby canine kidney cells

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