Optimization of L-phenylalanine production of Corynebacterium glutamicum under product feedback inhibition by elevated oxygen transfer rate

Chin Hang Shu, Chii Cherng Liao

研究成果: 雜誌貢獻期刊論文同行評審

21 引文 斯高帕斯(Scopus)

摘要

Production feedback inhibition both on cell growth and on product formation of phenylalanine fermentation might be alleviated by elevated oxygen supply. Batch fermentations by a high phenylalanine producing strain Corynebacterium glutamicum CCRC 18335 at various initial phenylalanine concentrations (P0) ranging from 0 to 20 g/L and different oxygen transfer rate coefficients (KLa) ranging from 23 to 76 h-1 were studied. The fermentation parameters with respect to P0 were strongly dependent on KLa. Cell yield favored higher KLa and lower P0. Product yield with respect to varying phenylalanine concentration was evaluated by the relative oxygen availability (ROA). The optimal ROA for phenylalanine formation was strongly dependent on the product concentration. While P0 was low, the product inhibition was less significant and the maximum product yield occurred while ROA was at 0.5-0.6. While P0 was high, the product inhibition was significant and the maximum product yield occurred while ROA was at 0.8-0.9. These results suggest that the product feedback inhibition of phenylalanine fermentation processes can be alleviated by a gradual increase in oxygen supply rate while the increasing product concentration is taken into account. The strategy is demonstrated in a fed-batch culture with elevated oxygen supply. The final phenylalanine concentration was 23.2 g/L, which was 45% better than that of the fed-batch fermentation without elevated oxygen supply. Likewise, the maximum productivity was improved by 42% at 0.37 g/(L · h).

原文???core.languages.en_GB???
頁(從 - 到)131-141
頁數11
期刊Biotechnology and Bioengineering
77
發行號2
DOIs
出版狀態已出版 - 20 1月 2002

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