On-chip SERS analysis for single mimic pathogen detection using Raman-labeled nanoaggregate-embedded beads with a dielectrophoretic chip

Chen Han Huang, Hsing Ying Lin, I. Ting Kuo, Wen Hsin Hsieh, Ping Ji Huang, Tzyy Schiuan Yang, Lai Kwan Chau

研究成果: 書貢獻/報告類型會議論文篇章同行評審

摘要

The integration of Raman-labeled nanoaggregate-embedded beads (NAEBs) for high performance SERS analysis of single mimic pathogen on a self-designed dielectrophoretic chip is demonstrated. The Raman tags called NAEBs are silica-coated, dye-induced aggregates of a small number of gold nanoparticles (AuNPs). In this work, NAEBs consisting of a Raman dye tetramethyl-rhodamine-5- isothiosyanate (TRITC) are chemically functionalized with streptavidin to detect biotin-functionalized polystyrene (PS) microspheres which mimic as pathogens. The sample solution of completely mixed streptavidin-functionalized NAEBs and biotin-functionalized PS microspheres is pumped into the microfluidic channel of a dielectrophoretic chip. By giving an AC voltage on the embedded electrodes, a single mimic pathogen can be caught via the non-contact dielectrophoretic force and suspended at the central cross of four aluminum electrodes for subsequent Raman spectroscopic detection. The SERS signal of TRITC is used as a spectral signature of specific mimic pathogen recognition, otherwise only the background Raman signal of a PS microsphere is observed. A pathogen-specific biosensor based on the dielectrophoresis-Raman spectroscopy system is developed, and the proof-ofconcept is confirmed by the specific molecular interaction model of streptavidin with biotin. Therefore, the on-chip multiplex SERS analysis of pathogens can be anticipated by employing different dye-tagged NAEBs simultaneously in a sample solution. We believe this bioassay has the ability to screen and detect multiple pathogens with minimal sample processing and handling even a small number of pathogens is present.

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主出版物標題Third Asia Pacific Optical Sensors Conference
DOIs
出版狀態已出版 - 2012
事件3rd Asia Pacific Optical Sensors Conference - Sydney, NSW, Australia
持續時間: 31 1月 20123 2月 2012

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
8351
ISSN(列印)0277-786X

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???event.eventtypes.event.conference???3rd Asia Pacific Optical Sensors Conference
國家/地區Australia
城市Sydney, NSW
期間31/01/123/02/12

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