Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin; Shao Wen Wu; Ya Ching Chou; Jiun Han Lin; Ya Chi Huang; Mei Ru Chen; Nianhan Ma; Ching Hwa Tsai

doi:10.1016/j.virol.2015.02.015

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

Sue Jane Lin, Shao Wen Wu, Ya Ching Chou, Jiun Han Lin, Ya Chi Huang, Mei Ru Chen, Nianhan Ma, Ching Hwa Tsai

生醫科學與工程學系

研究成果: 雜誌貢獻 › 期刊論文 › 同行評審

9 引文斯高帕斯（Scopus）

摘要

Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

原文	???core.languages.en_GB???
頁（從 - 到）	24-33
頁數	10
期刊	Virology
卷	481
DOIs	https://doi.org/10.1016/j.virol.2015.02.015
出版狀態	已出版 - 1 7月 2015

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10.1016/j.virol.2015.02.015

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title = "Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival",

abstract = "Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.",

keywords = "Apoptosis, EBV, LCL, TIMP-1, Zta",

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T1 - Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

AU - Lin, Sue Jane

AU - Wu, Shao Wen

AU - Chou, Ya Ching

AU - Lin, Jiun Han

AU - Huang, Ya Chi

AU - Chen, Mei Ru

AU - Ma, Nianhan

AU - Tsai, Ching Hwa

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

AB - Epstein Barr virus (EBV) uses various strategies to manipulate host cytokine production in favor of the survival of infected B-cells. Microarray and cytokine protein array assays revealed that tissue inhibitor of metalloproteinase-1 (TIMP-1) was significantly up-regulated in EBV-infected primary B cells and maintained in abundance in EBV-immortalized lymphoblastoid cell lines (LCLs). TIMP-1 plays critical roles in extracellular matrix homeostasis and regulates signaling pathways. In this study, we demonstrated that the EBV-encoded immediate early lytic protein, Zta, upregulates mainly TIMP-1 expression by binding to the AP-1 site within the TIMP-1 promoter. Moreover, knockdown of TIMP-1 expression promoted cisplastin and cold shock-induced death of LCLs. This study provides a mechanistic link between EBV-induced TIMP-1 expression and its impact on LCL survival.

KW - Apoptosis

KW - EBV

KW - LCL

KW - TIMP-1

KW - Zta

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DO - 10.1016/j.virol.2015.02.015

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AN - SCOPUS:84924296960

SN - 0042-6822

VL - 481

SP - 24

EP - 33

JO - Virology

JF - Virology

ER -

Novel expression and regulation of TIMP-1 in Epstein Barr virus-infected cells and its impact on cell survival

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