Noninvasive intravital cellular diagnosis of atopic dermatitis by using harmonic optical virtual biopsy

Szu Yu Chen, Jyh Hong Lee, Bor Luen Chiang, Chi Kuang Sun

研究成果: 書貢獻/報告類型會議論文篇章同行評審


Atopic dermatitis (AD) is now very common in people who live in cities, especially for babies and children. Since the cause of AD is still not completely understood and each person may have his own mixed symptoms that can change over time, diagnosis of AD can not be done precisely. Unlike some skin diseases, physical biopsy is rarely used in diagnosing AD on account of its low urgency. Thus, only indirect diagnoses, like asking for a medical history to learn about the symptoms and to rule out other diseases can be carried out. To gain insight into cellular details of AD for long-term diagnosing without physical biopsy, a noninvasive in vivo tool with a sub-micron subsurface resolution and high penetrability has to be used. In this presentation, we show that harmonic optical virtual biopsy can provide the required noninvasive cellular imaging, and is ideal for future clinical diagnosis of AD. Harmonic optical microscopy has been demonstrated to have the capability to reveal cellular morphology of human skin from epidermis to dermis layer. Third harmonic generation (THG), which is sensitive to inhomogeneous interfaces, can show the structures of skins, and can be used to reveal the morphological changes, for example, the thicken cuticle which is a common symptom of AD. Second harmonic generation (SHG), which occurs in non-centrosymmetric structures, has excellent contrast in collagen fibers and can show the pathological changes of dermis layer. Utilizing both THG and SHG, useful information may be given to facilitate the diagnosis of AD.

主出版物標題Photonic Therapeutics and Diagnostics III
出版狀態已出版 - 2007
事件Photonic Therapeutics and Diagnostics III - San Jose, CA, United States
持續時間: 20 1月 200721 1月 2007


名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE


???event.eventtypes.event.conference???Photonic Therapeutics and Diagnostics III
國家/地區United States
城市San Jose, CA


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