Non-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution

Szu Yu Chen, Chiao Sheng Lu, Chia Hua Yeh

研究成果: 雜誌貢獻期刊論文同行評審

10 引文 斯高帕斯(Scopus)

摘要

A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.

原文???core.languages.en_GB???
頁(從 - 到)338-347
頁數10
期刊Biomedical Optics Express
5
發行號2
DOIs
出版狀態已出版 - 2014

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