Nanoparticle based simple electrochemical biosensor platform for profiling of protein-nucleic acid interactions

Yifan Dai, Liang Yuan Chiu, Yongkun Sui, Quanbin Dai, Srinivasa Penumutchu, Niyati Jain, Liming Dai, Christian A. Zorman, Blanton S. Tolbert, R. Mohan Sankaran, Chung Chiun Liu

研究成果: 雜誌貢獻期刊論文同行評審

19 引文 斯高帕斯(Scopus)

摘要

The analysis of protein-nucleic acid interactions is essential for biophysics related research. However, simple, rapid, and accurate methods for quantitative analysis of biomolecular interactions are lacking. We herein establish an electrochemical biosensor approach for protein-nucleic acid binding analysis. Nanoparticle based sensors are fabricated by highly-controlled inkjet printing followed by plasma conversion. A novel bioconjugation method is demonstrated as a simple and rapid approach for protein-based biosensor fabrication. As a proof of concept, we analyzed the binding interaction between unwinding protein 1 (UP1) and SL3ESS3 RNA, confirming the accuracy of this nanoparticle based electrochemical biosensor approach with traditional biophysical methods. We further accurately profiled and differentiated a unique binding interaction pattern of multiple G-tract nucleic acid sequences with heterogeneous nuclear ribonucleoprotein H1. Our study provides insights into a potentially universal platform for in vitro biomolecule interaction analysis using a nanoparticle based electrochemical biosensor approach.

原文???core.languages.en_GB???
頁(從 - 到)46-54
頁數9
期刊Talanta
195
DOIs
出版狀態已出版 - 1 4月 2019

指紋

深入研究「Nanoparticle based simple electrochemical biosensor platform for profiling of protein-nucleic acid interactions」主題。共同形成了獨特的指紋。

引用此