@article{4bb764b7ed8b4a56be4c4844941f5353,
title = "Monitoring the polysaccharide quality of Agaricus blazei in submerged culture by examining molecular weight distribution and TNF-α release capability of macrophage cell line RAW 264.7",
abstract = "A method for monitoring the biological activity of broth polysaccharides of Agaricus blazei (AB-BP) in a submerged culture is described. The TNF-α releasing capability of AB-BP on the macrophage cell line, RAW 264.7, correlated with the molecular weight of AB-BP. The quality of polysaccharide in a submerged culture of Agaricus blazei was indirectly monitored by analyzing the distribution of its molecular weight within 1 h. The harvest time of the maximum polysaccharide production did not coincide with that of the maximum biological characteristics in the batch culture.",
keywords = "Agaricus blazei, Molecular weight, Polysaccharide, Quality control, Tumor necrosis factor-α",
author = "Shu, {Chin Hang} and Wen, {Bor Jiun} and Lin, {Ko Jung}",
note = "Funding Information: The authors would like to thank the Council of Agriculture of the Republic of China, Taiwan, for financially supporting this research under Contract No. COA 90 AG-3.1.3-FD-Z3.",
year = "2003",
month = dec,
doi = "10.1023/B:BILE.0000007066.00221.b9",
language = "???core.languages.en_GB???",
volume = "25",
pages = "2061--2064",
journal = "Biotechnology Letters",
issn = "0141-5492",
number = "24",
}