Monitoring DNA hybridization with a simply manufactured GMR biosensor

In this letter, a real-time approach to identify the various DNA molecules hybridized of the integrated micro-fluid with the simplest manufactured guided-mode resonance (GMR) sensor is presented. By monitoring the resonant peak wavelength shift of GMR biosensor, the DNA hybridization experiment can be recognized and clarified. The biosensor exhibited sensitivities about 2.14nm in peak wavelength shift for the detection of the hybridizing of both the capture DNA and the probe DNA. Furthermore, the stability and reliability of nucleic acid hybridization on the GMR sensor are examined by combining the sensor with the fluidic elements. The results reveal that the dynamics of DNA hybridization on the GMR sensor can obviously be monitored in real time.