Mirror-based broadband scanner with minimized aberration

Jiun Yann Yu, Yu Yi Tzeng, Chen Han Huang, Hsiang Chen Chui, Shi Wei Chu

研究成果: 書貢獻/報告類型會議論文篇章同行評審


To obtain specific biochemical information in optical scanning microscopy, labeling technique is routinely required. Instead of the complex and invasive sample preparation procedures, incorporating spectral acquisition, which commonly requires a broadband light source, provides another mechanism to enhance molecular contrast. But most current optical scanning system is lens-based and thus the spectral bandwidth is limited to several hundred nanometers due to anti-reflection coating and chromatic aberration. The spectral range of interest in biological research covers ultraviolet to infrared.For example, the absorption peak of water falls around 3 μm, while most proteins exhibit absorption in the UV-visible regime. For imaging purpose, the transmission window of skin and cerebral tissues fall around 1300 and 1800 nm, respectively. Therefore, to extend the spectral bandwidth of an optical scanning system from visible to mid-infrared, we propose a system composed of metallic coated mirrors. A common issue n such a mirror-based system is aberrations induced by oblique incidence.We propose to compensate astigmatism by exchanging the sagittal and tangential planes of the converging spherical mirrors in the scanning system. With the aid of an optical design software, we build a diffraction-limited broadband scanning system with wavefront flatness better than ?/4 at focal plane. Combined with a mirror-based objective this microscopic system will exhibit full spectral capability and will be useful in microscopic imaging and therapeutic applications.

主出版物標題Single Molecule Spectroscopy and Imaging II
出版狀態已出版 - 2009
事件Single Molecule Spectroscopy and Imaging II - San Jose, CA, United States
持續時間: 24 1月 200925 1月 2009


名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE


???event.eventtypes.event.conference???Single Molecule Spectroscopy and Imaging II
國家/地區United States
城市San Jose, CA


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