Microfluidic systems using localized molecular imprinting polymers for detection of nano-scale bio-molecules based on surface plasmon resonance

This paper reports a new microfluidic system integrated with localized molecular imprinting polymers (MIP) for detection of nano-scale bio-molecules based on surface plasmon resonance (SPR). The label-free bio-sensing method incorporates SPR biosensing and an innovative microfluidic biochip capable of transporting a specific amount of bio-samples inside multiple microchannels using micropumps to sensing regions where multiple MIP films are locally spun-coated such that highly-sensitive, high-throughput, and highly-specific bio-sensing of nano-scale bio-molecules could be achieved. The microfluidic SPR/MIP biochips have the potential to be widely used for nano bio-sensing applications. While compared to large-scale SPR techniques, the developed microfluidic system has a comparable sensitivity with less consumption of samples and can perform the sensing in an automatic format.