Microcalorimetric studies of the interactions of lysozyme with immobilized metal ions: Effects of ion, pH value, and salt concentration

Fu Yung Lin, Wen Yih Chen, Lung Ching Sang

研究成果: 雜誌貢獻期刊論文同行評審

41 引文 斯高帕斯(Scopus)

摘要

This study extends previous research on the interaction of biomaterials with immobilized Cu(II) by isothermal titration calorimetry (ITC) on Fe(III). The difference of the binding behavior of protein with that of the immobilized metal ions is also discussed. For the immobilized Fe(III), ITC results show that the adsorption enthalpy at a constant pH value decreased as the NaCl concentration increased and also decreased with the pH values at constant NaCl concentrations. The adsorption enthalpy become negative under higher pH values or higher salt concentrations indicating the adsorption process is partly driven by the enthalpy. The enthalpy of lysozyme with Fe(III) is higher than that with Cu(II) implying that the heat required for the dehydration of Cu(II) is lower than for the dehydration of Fe(III) and/or that the heat generated from the formation of the coordination with Cu(II) is higher than with Fe(III). In addition, the comparison of different immobilized metal ions corresponding to the equilibrium binding affinity suggests that the binding force of lysozyme with Cu(II) is higher than with Fe(III). This study presents the chemical differences between the binding affinity and the adsorption enthalpy of lysozyme interacting with the immobilized metal ions. The binding and thermodynamic data presented in this study elucidate the mechanism and process of lysozyme binding with immobilized metal ions. In addition, the thermodynamic characteristic functions provide valuable information enabling a more thorough understanding of protein adsorption at the immobilized metal ion affinity surface.

原文???core.languages.en_GB???
頁(從 - 到)373-379
頁數7
期刊Journal of Colloid and Interface Science
214
發行號2
DOIs
出版狀態已出版 - 15 6月 1999

指紋

深入研究「Microcalorimetric studies of the interactions of lysozyme with immobilized metal ions: Effects of ion, pH value, and salt concentration」主題。共同形成了獨特的指紋。

引用此