Microcalorimetric studies of the interactions of imidazole with immobilized Cu(II): Effects of pH value and salt concentration

Ching Fa Wu, Wen Yih Chen, Jiunn Fwu Lee

研究成果: 雜誌貢獻期刊論文同行評審

29 引文 斯高帕斯(Scopus)

摘要

In this investigation, we measured the influence of pH value and salt concentration on the heat of interaction between imidazole and CS-IDA-Cu(II) gel with a highly sensitive microcalorimeter. The direct enthalpy measurement of the interaction provides thermodynamic information regarding the binding behavior of imidazole toward the immobilized metal ion. The changes in binding enthalpy with the adsorbed amount of imidazole measured at various pH values and salt concentrations are discussed and the binding isotherm is reported. The binding and thermodynamic data obtained in this study can provide information on the mechanism and process of imidazole and protein binding with immobilized metal ions.

原文???core.languages.en_GB???
頁(從 - 到)236-242
頁數7
期刊Journal of Colloid and Interface Science
183
發行號1
DOIs
出版狀態已出版 - 15 10月 1996

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