Measurements of lactate concentration using lactate oxidase and an electrochemical oxygen sensor

Lactate oxidase was used in combination with an electrochemical dissolved oxygen sensor to measure L‐lactate concentration in the physiological saline solution. The rate of oxygen consumption was found to have an excellent linear relationship with the lactate concentration on a log‐log scale in the lactate concentration range of 0.3–25 mM. This detection does not require additional reagents and can be developed into a simple method of L‐lactate determination. The effects of the temperature and pH of the solution on the reaction rate were investigated and discussed.