TY - JOUR
T1 - Mangrove Helps
T2 - Sonneratia alba-Synthesized Silver Nanoparticles Magnify Guppy Fish Predation Against Aedes aegypti Young Instars and Down-Regulate the Expression of Envelope (E) Gene in Dengue Virus (Serotype DEN-2)
AU - Murugan, Kadarkarai
AU - Dinesh, Devakumar
AU - Paulpandi, Manickam
AU - Subramaniam, Jayapal
AU - Rakesh, R.
AU - Amuthavalli, Pandiyan
AU - Panneerselvam, Chellasamy
AU - Suresh, Udaiyan
AU - Vadivalagan, Chitravel
AU - Alsalhi, Mohamad Saleh
AU - Devanesan, Sandhanasamy
AU - Wei, Hui
AU - Higuchi, Akon
AU - Nicoletti, Marcello
AU - Canale, Angelo
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2016, Springer Science+Business Media New York.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The control of dengue vectors with effective tools is crucial. Here, we fabricated silver nanoparticles (AgNP) using a cheap method relying to a mangrove extract (Sonneratia alba) as a reducing and stabilizing agent. AgNP were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. LC50of S. alba extract against Aedes aegypti ranged from 192.03 ppm (larva I) to 353.36 ppm (pupa). LC50of AgNP ranged from 3.15 (I) to 13.61 ppm (pupa). Sub-lethal doses of AgNP magnified predation rates of guppy fishes, Poecilia reticulata, against Ae. aegypti and Chironomus kiiensis larvae. Mangrove-fabricated AgNP were evaluated for their antimicrobial potential against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, using the agar disc diffusion and minimum inhibitory concentration protocol. Notably, S. alba-synthesized AgNP tested at doses ranging from 5 to 15 µg/mL down-regulated the expression of the envelope (E) gene and protein in dengue virus (serotype DEN-2), while only little cytotoxicity rates (i.e. <15%) were detected on Vero cells when AgNP were tested at 10 µg/mL. Overall, this study pointed out the potential of S. alba-synthesized AgNP to develop eco-friendly nanoformulations effective against dengue virus and its mosquito vectors.
AB - The control of dengue vectors with effective tools is crucial. Here, we fabricated silver nanoparticles (AgNP) using a cheap method relying to a mangrove extract (Sonneratia alba) as a reducing and stabilizing agent. AgNP were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. LC50of S. alba extract against Aedes aegypti ranged from 192.03 ppm (larva I) to 353.36 ppm (pupa). LC50of AgNP ranged from 3.15 (I) to 13.61 ppm (pupa). Sub-lethal doses of AgNP magnified predation rates of guppy fishes, Poecilia reticulata, against Ae. aegypti and Chironomus kiiensis larvae. Mangrove-fabricated AgNP were evaluated for their antimicrobial potential against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi, using the agar disc diffusion and minimum inhibitory concentration protocol. Notably, S. alba-synthesized AgNP tested at doses ranging from 5 to 15 µg/mL down-regulated the expression of the envelope (E) gene and protein in dengue virus (serotype DEN-2), while only little cytotoxicity rates (i.e. <15%) were detected on Vero cells when AgNP were tested at 10 µg/mL. Overall, this study pointed out the potential of S. alba-synthesized AgNP to develop eco-friendly nanoformulations effective against dengue virus and its mosquito vectors.
KW - Arbovirus
KW - Biosafety
KW - Green-synthesis
KW - Poecilia reticulata
KW - Sonneratia alba
UR - http://www.scopus.com/inward/record.url?scp=84994754044&partnerID=8YFLogxK
U2 - 10.1007/s10876-016-1115-7
DO - 10.1007/s10876-016-1115-7
M3 - 期刊論文
AN - SCOPUS:84994754044
SN - 1040-7278
VL - 28
SP - 437
EP - 461
JO - Journal of Cluster Science
JF - Journal of Cluster Science
IS - 1
ER -
