TY - JOUR
T1 - Magnetic nanoparticles are highly toxic to chloroquine-resistant Plasmodium falciparum, dengue virus (DEN-2), and their mosquito vectors
AU - Murugan, Kadarkarai
AU - Wei, Jiang
AU - Alsalhi, Mohamad Saleh
AU - Nicoletti, Marcello
AU - Paulpandi, Manickam
AU - Samidoss, Christina Mary
AU - Dinesh, Devakumar
AU - Chandramohan, Balamurugan
AU - Paneerselvam, Chellasamy
AU - Subramaniam, Jayapal
AU - Vadivalagan, Chithravel
AU - Wei, Hui
AU - Amuthavalli, Pandiyan
AU - Jaganathan, Anitha
AU - Devanesan, Sandhanasamy
AU - Higuchi, Akon
AU - Kumar, Suresh
AU - Aziz, Al Thabiani
AU - Nataraj, Devaraj
AU - Vaseeharan, Baskaralingam
AU - Canale, Angelo
AU - Benelli, Giovanni
N1 - Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - A main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. We investigated the toxicity of magnetic nanoparticles (MNP) produced by Magnetospirillum gryphiswaldense (strain MSR-1) on chloroquine-resistant (CQ-r) and sensitive (CQ-s) Plasmodium falciparum, dengue virus (DEN-2), and two of their main vectors, Anopheles stephensi and Aedes aegypti, respectively. MNP were studied by Fourier-transform infrared spectroscopy and transmission electron microscopy. They were toxic to larvae and pupae of An. stephensi, LC50 ranged from 2.563 ppm (1st instar larva) to 6.430 ppm (pupa), and Ae. aegypti, LC50 ranged from 3.231 ppm (1st instar larva) to 7.545 ppm (pupa). MNP IC50 on P. falciparum were 83.32 μg ml−1 (CQ-s) and 87.47 μg ml−1 (CQ-r). However, the in vivo efficacy of MNP on Plasmodium berghei was low if compared to CQ-based treatments. Moderate cytotoxicity was detected on Vero cells post-treatment with MNP doses lower than 4 μg ml−1. MNP evaluated at 2–8 μg ml−1 inhibited DEN-2 replication inhibiting the expression of the envelope (E) protein. In conclusion, our findings represent the first report about the use of MNP in medical and veterinary entomology, proposing them as suitable materials to develop reliable tools to combat mosquito-borne diseases.
AB - A main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. We investigated the toxicity of magnetic nanoparticles (MNP) produced by Magnetospirillum gryphiswaldense (strain MSR-1) on chloroquine-resistant (CQ-r) and sensitive (CQ-s) Plasmodium falciparum, dengue virus (DEN-2), and two of their main vectors, Anopheles stephensi and Aedes aegypti, respectively. MNP were studied by Fourier-transform infrared spectroscopy and transmission electron microscopy. They were toxic to larvae and pupae of An. stephensi, LC50 ranged from 2.563 ppm (1st instar larva) to 6.430 ppm (pupa), and Ae. aegypti, LC50 ranged from 3.231 ppm (1st instar larva) to 7.545 ppm (pupa). MNP IC50 on P. falciparum were 83.32 μg ml−1 (CQ-s) and 87.47 μg ml−1 (CQ-r). However, the in vivo efficacy of MNP on Plasmodium berghei was low if compared to CQ-based treatments. Moderate cytotoxicity was detected on Vero cells post-treatment with MNP doses lower than 4 μg ml−1. MNP evaluated at 2–8 μg ml−1 inhibited DEN-2 replication inhibiting the expression of the envelope (E) protein. In conclusion, our findings represent the first report about the use of MNP in medical and veterinary entomology, proposing them as suitable materials to develop reliable tools to combat mosquito-borne diseases.
KW - Antiviral activity
KW - Magnetospirillum gryphiswaldense
KW - Magnetotactic bacteria
KW - Malaria
KW - Yellow fever
KW - Zika virus
UR - http://www.scopus.com/inward/record.url?scp=84994242396&partnerID=8YFLogxK
U2 - 10.1007/s00436-016-5310-0
DO - 10.1007/s00436-016-5310-0
M3 - 期刊論文
C2 - 27815736
AN - SCOPUS:84994242396
SN - 0932-0113
VL - 116
SP - 495
EP - 502
JO - Parasitology Research
JF - Parasitology Research
IS - 2
ER -
