Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity

Akon Higuchi, Shih Hsuan Kao, Qing Dong Ling, Yen Ming Chen, Hsing Fen Li, Abdullah A. Alarfaj, Murugan A. Munusamy, Kadarkarai Murugan, Shih Chang Chang, Hsin Chung Lee, Shih Tien Hsu, S. Suresh Kumar, Akihiro Umezawa

研究成果: 雜誌貢獻期刊論文同行評審

45 引文 斯高帕斯(Scopus)

摘要

The tentative clinical application of human pluripotent stem cells (hPSCs), such as human embryonic stem cells and human induced pluripotent stem cells, is restricted by the possibility of xenogenic contamination resulting from the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. Therefore, we investigated hPSC cultures on biomaterials with different elasticities that were grafted with different nanosegments. We prepared dishes coated with polyvinylalcohol-co-itaconic acid hydrogels grafted with an oligopeptide derived from vitronectin (KGGPQVTRGDVFTMP) with elasticities ranging from 10.3 to 30.4 kPa storage moduli by controlling the crosslinking time. The hPSCs cultured on the stiffest substrates (30.4 kPa) tended to differentiate after five days of culture, whereas the hPSCs cultured on the optimal elastic substrates (25 kPa) maintained their pluripotency for over 20 passages under xeno-free conditions. These results indicate that cell culture matrices with optimal elasticity can maintain the pluripotency of hPSCs in culture.

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文章編號18136
期刊Scientific Reports
5
DOIs
出版狀態已出版 - 14 12月 2015

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