Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy

Xiang Yu Zhuang, Chao Kai Tseng, Chien Jung Lo

研究成果: 書貢獻/報告類型篇章同行評審

摘要

Bacterial flagella are molecular machines used for motility and chemotaxis. The flagellum consists of a thin extracellular helical filament as a propeller, a short hook as a universal joint, and a basal body as a rotary motor. The filament is made up of more than 20,000 flagellin molecules and can grow to several micrometers long but only 20 nanometers thick. The regulation of flagellar assembly and ejection is important for bacterial environmental adaptation. However, due to the technical difficulty to observe these nanostructures in live cells, our understanding of the flagellar growth and loss is limited. In the last three decades, the development of fluorescence microscopy and fluorescence labeling of specific cellular structure has made it possible to perform the real-time observation of bacterial flagellar assembly and ejection processes. Furthermore, flagella are not only critical for bacterial motility but also important antigens stimulating host immune responses. The complete understanding of bacterial flagellar production and ejection is valuable for understanding macromolecular self-assembly, cell adaptation, and pathogen-host interactions.

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主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面35-42
頁數8
DOIs
出版狀態已出版 - 2023

出版系列

名字Methods in Molecular Biology
2646
ISSN(列印)1064-3745
ISSN(電子)1940-6029

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